牛昱宇博士

“国家高层次人才”特聘教授项目入选者、科技部重点研发计划项目首席科学家

Yuyu Niu, Ph.D.

Dean & Professor of Faculty of Life Science and Technology, KUST

Associate Director and Professor of State Key Laboratory of Primate Biomedical Research

一、工作学习经历

2019年-，太阳集团tcy8722，教授，经理

2014年-，大阳集团娱乐网址8722灵长类转化医学研究院，教授

2007年-2014年，中国科学院昆明动物研究所，副研究员

2006年-2008年，德国柏林野生与笼养动物研究所，访问学者

2001年-2007年，中国科学院昆明动物研究所，博士

1995年-1999年，云南师范大学生命科学系，本科

二、研究领域

灵长类基因编辑；灵长类胚胎发育与疾病

Gene editing, Primate embryonic development

早期胚胎发育是哺乳动物生命发生的重要阶段，这一时期的关键生物学事件，如合子基因启动，表观遗传维持与重建，胚层分化和器官形成等等，是发育生物学的重要基础理论，又和人类健康和疾病密切相关。然而，我们对其中的诸多科学问题却知之甚少。本实验室结合灵长类早期胚胎（体外受精，单性生殖和体细胞核移植）培养，基因编辑，干细胞与示踪，单细胞组学和灵长类疾病动物模型等技术体系，系统研究灵长类细胞多能性的维持与重建，表观遗传维持与胚层分化的关系，退行性疾病的早期诊断和干预等等重要科学问题，以期揭示人类生命发生和疾病形成的奥秘。

Research in Yuyu Niu's laboratory is focused on early embryo development and reprogramming, particularly preimplantation embryo development which play an important role in cell differentiation and organ initiation, and therefore have a significant on human disease. Over the last decade, Dr. Niu has made significant contributions to non-human primate genetic engineering and early embryo development that include: 1) the first transgenic rhesus monkey in China (PNAS 2010); 2) the first monkey model with genome engineered by precision gene-targeting methods (Cell 2014; Cell 2017); 3) the continuous development of monkey embryos in vitro beyond early gastrulation and to 20 days post fertilization (Science 2019). Built on the strength in non-human primate research, the Yuyu Niu's laboratory has expanded its capability to use a variety of state-of-the-art techniques, including single cell transcriptomics, cell lineage tracing, stem cells, somatic cell nuclear transfer and CRISPR/Cas9-based genomic editing tounderstand the molecularevents at the beginning of primate life and cell reprogramming. In addition, the lab has studied on mechanism and biomarker of neurodegeneration diseases, such as Parkinson’s disease and Amyotrophic lateral sclerosis.

三、开设课程

细胞生物学、发育生物学

四、代表性论文（近5年）

1.Kang Y, D. S.,Zeng Y, Wang F, Yang P, Yang Z, Pu Y, Li Z, Chen X, Tian B, Si W, Ji W*, Niu Y*. Cloningand base editingof GFP transgenic rhesus monkey and off-target analysis.Science Advances,doi:10.1126/sciadv.abo3123(2022). (* co-corresponding author)

2.Zhu X, G. Y., Chu C, Liu D, Duan K, Yin Y, Si C, Kang Y, Yao J, Du X, Li J, Zhao S, Ai Z, Zhu Q, Ji W*, Niu Y*, Li T*. BRN2 as a key gene drives the early primate telencephalon developmentScience Advances, doi:10.1126/sciadv.abl7263 (2022). (* co-corresponding author)

3.Zhong T, W. J., Wu K, Chen L, Zhao F, Pei Y, Wang Y, Zhang H, Wu Z, Huang Y, Li T, Wang L, Chen Y, Ji W, Zhang Y, Li G*, Niu Y*. Longitudinal brain atlases of early developing cynomolgus macaques from birth to 48 months of age.Neuroimage247, 118799, doi:10.1016/j.neuroimage.2021.118799 (2022). (* co-corresponding author)

4.Yang R, G. A., Kang Y, Si C, Chu C, Zheng Y, Chen Z, Gruber PJ, Xiao Y, Zhou C, Witman N, Eroglu E, Leung CY, Chen Y, Fu J, Ji W*, Lanner F*, Niu Y*, Chien KR*. Amnion signals are essential for mesoderm formation in primates.Nature communications12, 5126 (2021). (* co-corresponding author)

5.Tan T*, W. J., Si C, Dai S, Zhang Y, Sun N, Zhang E, Shao H, Si W, Yang P, Wang H, Chen Z, Zhu R, Kang Y, Hernandez-Benitez R, Martinez Martinez L, Nuñez Delicado E, Berggren WT, Schwarz M, Ai Z, Li T, Rodriguez Esteban C, Ji W*, Niu Y*, Izpisua Belmonte JC*. Chimeric contribution of human extended pluripotent stem cells to monkey embryos ex vivo.Cell184, 2020-2032 e2014, doi:10.1016/j.cell.(2021). (* co-corresponding author)

6.Chu C, Z. W*. , Kang Y, Si C, Ji W*, Niu Y*, Zhang Y*. Analysis of developmental imprinting dynamics in primates using SNP-free methods to identify imprinting defects in cloned placenta.Developmental cell56, 2826-2840. e2827 (2021). (* co-corresponding author)

7.Chen ZZ, W. J., Kang Y, Yang QY, Gu XY, Zhi DL, Yan L, Long CZ, Shen B*, Niu YY*. PINK1 gene mutation by pair truncated sgRNA/Cas9-D10A in cynomolgus monkeys.Zoological Research42, 469 (2021). (* co-corresponding author)

8.Chen C, J. W., Niu Y*. Primate Organoids and Gene-Editing Technologies toward Next-Generation Biomedical Research.Trends in Biotechnology39(12):1332-1342 (2021).

9.Wang F, Z. W., Yang Q, Kang Y, Fan Y, Wei J, Liu Z, Dai S, Li H, Li Z, Xu L, Chu C, Qu J, Si C, Ji W*, Liu GH*, Long C*, Niu Y*. Generation of a Hutchinson–Gilford progeria syndrome monkey model by base editing.Protein & cell11, 809-824 (2020). (* co-corresponding author)

10.Li B, N. Y*., Ji W*, Dong Y*. Strategies for the CRISPR-based therapeutics.Trends in pharmacological sciences41, 55-65 (2020). (* co-corresponding author)

11.Wang D, N. Y*., Ren L, Kang Y, Tai PWL, Si C, Mendonca CA, Ma H, Gao G, Ji W. Gene Delivery to Nonhuman Primate Preimplantation Embryos Using Recombinant Adeno‐Associated Virus.Advanced Science6, 1900440 (2019). (* co-firstauthor)

12.Niu Y*, S. N., Li C, Lei Y, Huang Z, Wu J, Si C, Dai X, Liu C, Wei J, Liu L, Feng S, Kang Y, Si W, Wang H, Zhang E, Zhao L, Li Z, Luo X, Cui G, Peng G, Izpisúa Belmonte JC*, Ji W*, Tan T*. Dissecting primate early post-implantation development using long-term in vitro embryo culture.Science366 (2019). (* co-corresponding author)

13.Kang Y, C. C., Wang F, Niu Y*. CRISPR/Cas9-mediated genome editing in nonhuman primates.Disease models & mechanisms12, dmm039982 (2019).

五、主持项目（正在执行）

1.国家重点研发计划“发育编程及其代谢调节”项目，灵长类原肠发育与器官形成的命运决定调控机制，2021-2026，3100万元

2.国家自然科学基金联合基金项目，利用单碱基编辑技术构建早衰症猴模型及其机理研究，2022-2025，233万元

3.国家自然科学基金面上项目，肌萎缩侧索硬化症灵长类动物转基因模型的建立及早期发病机制研究，2021-2024，58万元

六、联系方式

地址：云南省昆明市呈贡区大学城景明南路727号太阳集团tcy8722

电话：0871-65952808

E-mail：niuyy@kust.edu.cn